Fast, Free, and Flexible Peptide and Protein Quantification with FlashLFQ

  • Posted on: 26 July 2024
  • By: mshort
TitleFast, Free, and Flexible Peptide and Protein Quantification with FlashLFQ
Publication TypeJournal Article
Year of Publication2023
AuthorsMilikin RJ, Shortreed MR, Scalf M, Smith LM
JournalMethods Mol Biol.
Volume2426
Pagination303-313
Other Numbersdoi: 10.1007/978-1-0716-1967-4_13
Abstract

The rapid and accurate quantification of peptides is a critical element of modern proteomics that has become increasingly challenging as proteomic data sets grow in size and complexity. We present here FlashLFQ, a computer program for high-speed label-free quantification of peptides and proteins following a search of bottom-up mass spectrometry data. FlashLFQ is approximately an order of magnitude faster than established label-free quantification methods and can quantify data-dependent analysis (DDA) search results from any proteomics search program. It is available as a graphical user interface program, a command line tool, a Docker image, and integrated into the MetaMorpheus search software.