New Tools to Decipher the Role of lncRNAs and Their Protein Interactomes in Hematopoiesis
03/01/2020 to 02/29/2024
The proposed research aims to elucidate the biological functions of lncRNAs and their associated proteins to discover GATA factor-dependent regulatory circuits and networks that control hematopoiesis.
Sequence-specific Hybridization Capture for Discovery of Proteoform–lncRNA Interactions in Prostate Cancer
01/01/2020 to 12/31/2024
The proposed research aims to identify lncRNA-protein complexes that correlate with tumor aggressiveness in human prostate cancer samples and quantify the constituent lncRNAs and associated proteins.
Novel Technologies for Protein Analysis
05/01/2018 to 04/30/2023
The goal of this project is the development of novel technologies for proteomics and biological mass spectrometry. The work includesfive interrelated areas: proteoform identification and quantification, identification and quantification of proteins that interact with specific RNA transcripts, detection and identification of fusion peptides, new strategies for the identification and localization of post-translational modifications, and sample-specific proteomic databases.
Novel NeuCode Tagging Reagents for Identification and Quantification of Intact Proteoforms in Cancer Tissues
03/14/2018 to 02/28/2021
The goal of this project is to make possible the identification and quantification of intact proteoforms in clinically relevant cancer tissue samples. This will both open a new realm of potential cancer biomarkers for early cancer detection and provide critical new information on the precise molecular forms of proteins present in cancer tissues. This information is essential to understanding, and eventually treating, cancer.