Novel Technologies for Protein Analysis
05/01/2018 to 04/30/2023
The major goals of this project are to focus on the development of novel technologies for proteomics and biological mass spectrometry. The work involves five interrelated areas: proteoform identification and quantification, identification and quantification of proteins that interact with specific RNA transcripts, detection of fusion peptides, new strategies for the identification and localization of post-translational modifications, and sample-specific proteomic databases.
Novel NeuCode Tagging Reagents for Identification and Quantification of Intact Proteoforms in Cancer Tissues
03/14/2018 to 02/28/2021
The major goals of this project are to make possible the identification and quantification of intact proteoforms in clinically relevant cancer tissue samples. This will both open a new realm of potential cancer biomarkers for early cancer detection and provide critical new information on the precise molecular forms of proteins present in cancer tissues. This information is essential to understanding, and eventually treating, cancer.
The Role of Sorcs1 and Sortilin in Diabetes Susceptibility
07/15/2015 to 06/30/2020
The major goals of this project are to elucidate a newly identified branch point that determines whether insulin is packaged in insulin granules, or is diverted towards degradation. We will characterize mutations in Sortilin and Sorcs1 that are associated with decreased insulin in humans and in mice. Insights from our work may provide clues to the development of novel therapeutic approaches aimed at preserving the insulin reserve of pancreatic β-cells.