MetaMorpheus

Caterpillar Body with M's as the wingsMetaMorpheus is a software tool that identifies peptides, proteins, and proteoforms from mass spectrometry fragmentation (MS/MS) data. It is capable of searching both bottom-up (peptide) and top-down (intact proteoform) data. Currently, five graduate students and several undergraduates work on improving MetaMorpheus, making it one of our most collaborative and dynamic projects. Often, the motivation for adding a feature to MetaMorpheus is the need to analyze new types of data that existing software simply cannot handle. We use computer programming as a tool to solve these problems, much like traditional chemical biology uses organic chemistry to probe biological systems. MetaMorpheus was specifically designed to identify the “dark matter” of proteomics; where most search programs attempt to identify mostly unmodified peptides, we provide the capability of identifying peptides with a wide range of post-translational modifications. Recently, we have added the ability to identify proteoforms, chemically crosslinked peptides, and isotopically-labeled peptides. We also focus on providing this tool to the proteomics community as a free, open-source, easy-to-use tool. We have a community of over 200 users, which grows every day. We are constantly adding new features to the software, and release new versions of MetaMorpheus every few weeks.

Getting Started

Software Update Options

How to set up a Search Task

Global PTM Discovery

Team Conversation with Ben Neely

How to Create a Calibration Task

Converting Mass Spectrometry Data Files with MSConvert to .mzML for use in MetaMorpheus

How to Run MetaMorpheus in the Linux, Mac and Windows Command Line

How to use .toml files in MetaMorpheus

MetaMorpheus Label Free Quantification for Proteomics Using FlashLFQ

Annotating Peptide and Proteoform Fragmentation Data with MetaDraw in MetaMorpheus

Understanding MetaMorpheus Output – AllQuantifiedProteinGroups.psmtsv

Understanding MetaMorpheus Output – AllPSMs.psmtsv

Support for MetaMorpheus Software – Wiki, Email, GitHub, YouTube and Twitter

Download Install and Simple Search in Windows GUI

MetaMorpheus: Multi-Protease Data Analysis

MetaMorpheus: Mass Spectra Files

MetaMorpheus: Quick Start Guide for the Windows GUI

MetaMorpheus: Protein Databases